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孙娟,闻静▲,王启之,张成斌,郑兆炜,邵明.长链非编码RNA H19在小鼠溃疡性结肠炎相关结直肠癌发病过程中的作用研究[J].中国医药科学,2024,14(11):25-28        基金项目:蚌埠医学院自然重点项目(2022byzd086)
长链非编码RNA H19在小鼠溃疡性结肠炎相关结直肠癌发病过程中的作用研究
Study on the role of long-chain noncoding RNA H19 in the pathogenesis of ulcerative colitis-related colorectal cancer in mice
  
DOI:
中文关键词:  溃疡性结肠炎;结直肠癌;长链非编码 RNA H19;信号传导及转录激活蛋白3;炎症
英文关键词:Ulcerative colitis; Colorectal cancer; Long-chain noncoding RNA H19; Signal transducer and activator of transcription-3; Inflammation
作者单位
孙娟,闻静▲,王启之,张成斌,郑兆炜,邵明 蚌埠医科大学第二附属医院消化内科,安徽蚌埠 233000 
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中文摘要:
      [摘要] 目的 探讨小鼠溃疡性结肠炎相关结直肠癌(CAC)发病过程中,长链非编码RNA H19(H19)的表达和意义。方法 将30只C57BL/6小鼠随机分成对照组和观察组。观察组小鼠用氧化偶氮甲烷/葡聚糖硫酸钠方式共同造模。对照组小鼠在相同时间给予腹腔内注射无菌生理盐水,同时饮用洁净水。第71天处死小鼠,比较两组小鼠结肠组织中H19、mRNA let-7a(let-7a)、p-Janus激酶2(p-JAK2)、p-转录激活因子3(p-STAT3)表达量的差异。结果 观察组小鼠结肠组织中H19 mRNA表达量高于对照组(P < 0.05),观察组小鼠结肠组织中let-7a mRNA表达量低于对照组(P < 0.05)。观察组小鼠结肠组织中p-JAK2和p-STAT3蛋白表达水平高于对照组(P < 0.05)。 结论H19在小鼠结肠癌组织中的高表达伴随着STAT3表达增加,对CAC发病起到了推动的作用。
英文摘要:
      [Abstract] Objective To explore the expression and significance of long-chain noncoding RNA H19 (H19) in the pathogenesis of ulcerative colitis-associated colorectal cancer (CAC) in mice. Methods A total of thirty C57BL/6 mice were randomly divided into the control group and the observation group. The model induced by azoxymethane and dextran sodium sulfate was established in the mice of the observation group. At the same time, mice in the control group were given intraperitoneal injection of sterile saline and were allowed to drink clean water. On the 71st day, the mice were killed, and the differences in the expressions of H19, microRNA let-7a (let-7a), phospho-Janus kinase 2 (p-JAK2) and phosphorylated signal transducer and activator of transcription-3 (p-STAT3) in the colon tissues of the two groups were compared. Results The expression of H19 in the colon of mice in the observation group was higher than that in the control group (P < 0.05), and the expression of let-7a in the colon tissue of mice in the observation group was lower than that in the control group (P < 0.05). The expression levels of p-JAK2 and p-STAT3 proteins in the colon of mice in the observation group were higher than those in the control group (P < 0.05). Conclusion The high expression of H19 in mouse colon cancer tissue is accompanied by the increase of STAT3 expression, which promotes the pathogenesis of CAC.
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