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钟茂林1,卓明1,李盈1,郭锐1,刘金龙1,张倩2.不同浓度的依托咪酯通过调控FEZF1-AS1的表达对结直肠癌细胞生物学功能的影响[J].中国医药科学,2024,14(11):17-20        基金项目:江西省卫生健康委科技计划项目(202130656);赣南医学院第一附属医院重点科技计划项目(YJZD202004)
不同浓度的依托咪酯通过调控FEZF1-AS1的表达对结直肠癌细胞生物学功能的影响
Impacts of different concentrations of etomidate on the biological function of colorectal cancer cells by regulating the expression of FEZF1-AS1
  
DOI:
中文关键词:  依托咪酯;结直肠癌;LncRNA FEZF1-AS1;miR-363-3p;凋亡
英文关键词:Etomidate; Colorectal cancer; LncRNA FEZF1-AS1; MiR-363-3p; Apoptosis
作者单位
钟茂林1,卓明1,李盈1,郭锐1,刘金龙1,张倩2 1.赣南医学院第一附属医院麻醉手术中心 江西省赣州市麻醉学重点实验室,江西赣州 341000;2.南京鼓楼医院集团宿迁医院麻醉科,江苏宿迁 223800 
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中文摘要:
      [摘要] 目的 探讨不同浓度依托咪酯对结直肠癌细胞生物学功能的影响,并分析其潜在机制。 方法 使用0、4、16、64 μmol/L依托咪酯分别处理结直肠癌细胞(LS513细胞)48 h,测定细胞存活率、凋亡率、迁移侵袭能力,并检测Bcl-2、BAX、Cleaved caspase-3及LncRNA FEZF1-AS1和miR-363-3p的表达水平。培养LS513细胞,分为三组:空白组、依托咪酯64μmol/L组、pcDNA-FEZF1-AS1组。比较三组细胞生物学功能。 结果 4、16、64 μmol/L依托咪酯处理LS513细胞后,细胞存活率、迁移细胞数、侵袭细胞数均降低,凋亡率增加,且凋亡相关蛋白Cleaved caspase-3、Bax表达升高,Bcl-2表达降低(P < 0.05);过表达LncRNA FEZF1-AS1后,LS513细胞LncRNA FEZF1-AS1表达升高,miR-363-3p表达降低,且过表达LncRNA FEZF1-AS1后LS513细胞存活率、迁移细胞数及侵袭细胞数升高,凋亡率降低(P < 0.05)。 结论 依托咪酯可抑制结直肠癌细胞迁移、侵袭,并促进其凋亡,且可能通过调控LncRNA FEZF1-AS1/miR-363-3p信号轴发挥作用。
英文摘要:
      [Abstract] Objective To investigate the impacts of different concentrations of etomidate on the biological function of colorectal cancer cells, so as to analyze its potential mechanism. Methods 0, 4, 16 and 64 μmol/L etomidate were used to treat colorectal cancer cells (LS513 cells) for 48 hours, the cell survival rate and apoptosis rate were determined, and the expression levels of Bcl-2, BAX, Cleaved caspase-3, LncRNA FEZF1-AS1 and miR-363-3p were detected. The cultured LS513 cells were divided into the blank group, the 64 μmol/L etomidate group and the pcDNA-FEZF1-AS1 group. The biological functions of the three groups of cells were compared. Results After treating LS513 cells with etomidate at 4, 16, 64 μmol/L, the cell survival rate, the number of migrating cells and the number of invading cells all decreased, the apoptosis rate increased, and the expression of apoptosis-related proteins Cleaved caspase-3 and Bax increased, while the expression of Bcl-2 decreased (P < 0.05). After overexpression of LncRNA FEZF1-AS1 in LS513 cells, the expression of LncRNA FEZF1-AS1 increased, and the expression of miR-363-3p decreased. In addition, after overexpression of LncRNA FEZF1-AS1, the survival rate, the number of migrating cells and the number of invading cells increased and the apoptosis rate decreased (P < 0.05). Conclusion Etomidate can inhibit the migration and invasion of colorectal cancer cells, and promote their apoptosis, and may play a role by regulating LncRNA FEZF1-AS1/miR-363-3p signal axis.
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