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谭劲,岳金宝,罗玉姣,吴丹,刘寻,李群.丹玄口康对ANE诱导的口腔黏膜下纤维化TGFβ1/Smad信号通路的影响[J].中国医药科学,2018,(5):27-31        基金项目:国家自然科学基金(81373701)
丹玄口康对ANE诱导的口腔黏膜下纤维化TGFβ1/Smad信号通路的影响
Effect of Danxuan Koukang on ANE induced TGF beta 1 /Smad signaling pathway in oral submucosal fibrosis
  
DOI:
中文关键词:  丹玄口康;口腔黏膜下纤维化;Ⅰ型胶原蛋白;TGFβ1/Smad 信号通路
英文关键词:Danxuan Koukang;Oral submucosal fibrosis;Type I collagen;TGF beta 1/Smad signal pathway
作者单位
谭劲,岳金宝,罗玉姣,吴丹,刘寻,李群 1. 湖南中医药大学第一附属医院;2. 湖南中医药大学研究生院 
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中文摘要:
      目的 探讨丹玄口康对 ANE 诱导的口腔黏膜下纤维化 TGFβ1/Smad 信号通路的影响。 方法 体外培养大鼠口腔黏膜 FB,以 ANE 为诱导剂,丹玄口康药物血清为干预物,以 TGFβ1/Smad 信号通路阻断剂LY2109761 为阳性对照物,采用免疫细胞化学法检测细胞内Ⅰ型胶原蛋白、磷酸化 Smad2/3 蛋白的表达变化,用酶联免疫吸附剂测定法检测培养上清Ⅰ型胶原的含量,采用荧光定量 PCR 检测Ⅰ型胶原 mRNA 的表达变化。 结果 ANE 刺激 FB 后,细胞和上清液中Ⅰ型胶原的表达、Smad2/3 的磷酸化胞核的转位水平和Ⅰ型胶原 mRNA 的表达均高于正常组(P < 0.05);ANE 刺激 FB 的同时给予 LY2109761 干预后,细胞和上清液中Ⅰ型胶原的表达、磷酸化 Smad2/3 的胞核转位水平和Ⅰ型胶原 mRNA 的表达均有所降低(P < 0.05);ANE 刺激 FB 的同时给予丹玄口康含药血清干预后,细胞和上清液中 I 型胶原的表达、磷酸化 Smad2/3 的胞核转位水平和 I 型胶原 mRNA 的表达均有所降低(P < 0.05)。 结论 丹玄口康可通过调控 TGFβ1/Smads 信号转导通路,抑制 ANE 刺激的 FB 胶原蛋白的合成,从而发挥抗纤维化作用。
英文摘要:
      Objective To explore the effect of Danxuan Koukang on ANE induced TGF beta 1/Smad signaling pathway in Oral Submucosal Fibrosis. Methods Oral submucosal FB of rats was cultured in vitro,and ANE was used as an inducer.The serum of Danxuan Koukang was taken as intervention,and TGF beta 1/Smad signal pathway blocker LY2109761 was used as a positive control.The expression of type Ⅰ collagen and phosphorylated Smad2/3 protein in cells was detected by immunocytochemistry.The content of type Ⅰ collagen in culture supernatant was detected by enzyme-linked immunosorbent assay,and the expression of collagen type I mRNA was detected by real-time quantitative PCR. Results After ANE stimulated FB,the expression of type I collagen,the translocation of Smad2/3 phosphorylated nucleus and the expression of type I collagen mRNA in the cells and supernatants were higher than those in the normal group (P < 0.05).When ANE stimulated FB and was intervened by LY2109761,the expression of type Ⅰ collagen, the nuclear translocation level of phosphorylated Smad2/3 and the expression of collagen mRNA of type Ⅰ in the cells and supernatants were decreased (P < 0.05).ANE stimulated FB and intervened by the serum of Danxuan Koukang.After the intervention,the expression of Ⅰ collagen,the nuclear translocation level of phosphorylated Smad2/3 and the expression of I type collagen mRNA in the cells and supernatants were decreased (P < 0.05). Conclusion Danxuan Koukang can inhibit the synthesis of FB collagen stimulated by ANE by regulating the TGF beta 1/Smads signal transduction pathway, thus exerting anti fibrosis effect.
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